Total RNA-seq

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For ordering information, sample requirements, and shipping instructions, download this PDF.

  • Preserved Cells

    Total RNA from cells or tissues preserved in RNAlater™, or frozen on dry ice, will be purified using mirVana™ technology and quality checked on a Agilent Bioanalyzer™.

  • Total RNA

    Total RNA may be further purified by your choice of RiboMinus™ or Poly(A) enrichment prior to cDNA synthesis.

  • cDNA

    Using Illumina’s TruSeq kit or Solid’s RNA-Seq kit RNA will be fragmented and cDNA synthesized for library construction.

  • Library Constructed

    Using Illumina™ or Applied Biosystems protocol, paired end or multiplexed libraries are generated for loading onto the appropriate platform.

  • Sequencing

    Using Illumina™ or Applied Biosystems™ reagents and instrument your samples are sequenced to a base pair and tag level specified by you.

  • Data Analysis

    Using our analysis pipeline raw data is analyzed for quality and base called. Tag data is mapped to a supported genome and made available for secure download or placed on your hard drive.

  • Genome Browser

    For quick viewing your NextGen data is loaded on a password protected mirror of the UCSC genome browser and available for viewing within hours.