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Total RNA from cells or tissues preserved in RNAlater™, or frozen on dry ice, will be purified using mirVana™ technology and quality checked on a Agilent Bioanalyzer™.
Total RNA may be further purified by your choice of RiboMinus™ or Poly(A) enrichment prior to cDNA synthesis.
Using Illumina’s TruSeq kit or Solid’s RNA-Seq kit RNA will be fragmented and cDNA synthesized for library construction.
Using Illumina™ or Applied Biosystems protocol, paired end or multiplexed libraries are generated for loading onto the appropriate platform.
Using Illumina™ or Applied Biosystems™ reagents and instrument your samples are sequenced to a base pair and tag level specified by you.
Using our analysis pipeline raw data is analyzed for quality and base called. Tag data is mapped to a supported genome and made available for secure download or placed on your hard drive.
For quick viewing your NextGen data is loaded on a password protected mirror of the UCSC genome browser and available for viewing within hours.